Total Internal Reflection Fluorescence microscopy accessory
Mad City Labs MicroMirror TIRF
The Mad City Labs' MicroMirror TIRF system is an enabling technology for advanced study of complex biomolecular interactions. MicroMirror TIRF is a proven design and offers a simple yet flexible instrument platform for single molecule research.
Integrated imaging & motion control platform
Adjustable micromirror mounts
TIRF lock feedback control
Integrated slide holder
Optical pathway accessibility
Robust & flexible platform
The technique of micro-mirror TIRF (total internal reflection fluorescence) microscopy is the only proven method to study the ordered assembly and function of multi-component biomolecular machines. The Mad City Labs' MicroMirror TIRF system is part of the single molecule imaging instrument portfolio, and draws on their expertise with high precision, high stability nanopositioning systems and microscopy solutions.
The MicroMirror TIRF system uses through-the-objective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. The elimination of the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment.
Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accomodate in conventional dichroic TIRF instruments. Each micro-mirror is mounted to a 6-axis precision mount allowing the user to make fine adjustments to the optical pathway. The imaging and motion control platform is designed to maximise optical pathway accessibility and flexibility, while also simplifying the optical alignment.
An advantage of the MicroMirror TIRF system is the open access to the entry and exit optical pathways. The motion control platform comprises an XY micropositioning platform with an integrated XYZ closed loop nanopositioning system. The XYZ closed loop nanopositioning uses proprietary PicoQ® sensors for sub-nanometer precision and high stability. A Z-axis micropositioner with integrated sample holder is mounted above the nanopositioning system.
Completing theMicroMirror TIRF system is the TIRF Lock feedback control consisting of a QPD sensor, TIRF Lock controller and software. The TIRF Lock module maintains the TIR signal through software feedback to the nanopositioner.
Imaging & Motion Control
Ranges of motion (XY)
Range of motion (Z)
Ranges of motion (XYZ)
Resonant Frequency (XYZ)
350 Hz ±15%
Recommended max. load (horizontal)
0 to 10V
TIRF Lock Feedback
A number of articles have been published by scientists around the globe who have used a Mad City Labs Micromirror TIRF within their research. Here is an example: