alpha300 R Raman Microscope












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- Model: WITec alpha300 R
Confocal Raman Imaging Microscope
WITec alpha300 R Raman Microscope
Features
- High speed, high resolution confocal Raman imaging
- Hyperspectral image generation
- Excellent diffraction limited lateral resolution of 200 - 300 nm
- Outstanding depth resolution for 3D image generation and depth profiles
- Non-destructive imaging technique: no staining of fixation of the sample required
- Ultra-high throughput lens-based spectroscopic system...
...highest sensitivity and best performance in spectral resolution
WITec's alpha300 R is the pre-eminent confocal Raman imaging system on the market today, setting the benchmark in terms of flexibility, sensitivity, speed and performance. With an excellent diffraction limited lateral resolution of between two and three hundred nanometres, plus outstanding depth resolution, it is ideally suited for 3D image generation and depth profiles.
The alpha300 R rapidly delivers hyperspectral image generation with the information of a complete Raman spectrum for every image pixel. Integration time is only 0.76 milliseconds per spectrum if the ultra-fast imaging option is chosen.
Operation Modes
- Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
- Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
- Fibre-coupled UHTS spectrometer specifically designed for Raman and low light applications
- Image stacks: 3D confocal Raman imaging
- Time series
- Single point Raman spectrum acquisition
- Single-point depth profiling
- Confocal Fluorescence Microscopy
- Bright Field Microscopy
Microscope specification
- Research grade optical microscope with 6x objective turret
- CCD camera video system
- LED white-light source for Köhler illumination
- Manual sample positioning in x- and y-direction
- Fibre coupling
- Sample size: 120 mm (x- & y-axes), 25 mm in z-axis
Ongoing development ensures the alpha300 R microscope is at the forefront of the technology.

Additional lasers
Additional UHTS-spectrometers (UV, VIS, NIR)
Automated sample positioning and measuring
Automated confocal Raman imaging
Automated multi-area and multi-point measurements
Full automation: see apyron
Ultrafast Raman imaging upgrade
Epi-fluorescence option
High sample adaptor
TrueSurface for Raman depth profiling
Dark Field Microscopy
Phase Contrast Microscopy
DIC option
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A number of papers have been published by scientists around the globe who have used a WITec Raman Microscope Imaging System within their research. Here are some examples:
- How dangerous is microplastic? Dr. Natalia Ivleva of TUM discusses her research
- Micro-Raman Imaging of Ferroelectric Domain Structures in the Bulk of PMN-PT Single Crystals
- Boosting third-harmonic generation by a mirror-enhanced anapole resonator
- Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics
- Highly efficient all-optical switching of magnetization in GdFeCo microstructures by interference-enhanced absorption of light