MicroMirror TIRF System - Mad City Labs

MicroMirror TIRF System - Mad City Labs

Total Internal Reflection Fluorescence microscopy accessory   

Mad City Labs MicroMirror TIRF   

The Mad City Labs' MicroMirror TIRF system is an enabling technology for advanced study of complex biomolecular interactions. MicroMirror TIRF is a proven design and offers a simple yet flexible instrument platform for single molecule research.

  • Integrated imaging & motion control platform
  • Adjustable micromirror mounts
  • TIRF lock feedback control
  • Integrated slide holder
  • Optical pathway accessibility
  • Robust & flexible platform

The technique of micro-mirror TIRF (total internal reflection fluorescence) microscopy is the only proven method to study the ordered assembly and function of multi-component biomolecular machines. The Mad City Labs' MicroMirror TIRF system is part of the single molecule imaging instrument portfolio, and draws on their expertise with high precision, high stability nanopositioning systems and microscopy solutions.

The MicroMirror TIRF system uses through-the-objective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. The elimination of the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment.

Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accomodate in conventional dichroic TIRF instruments. Each micro-mirror is mounted to a 6-axis precision mount allowing the user to make fine adjustments to the optical pathway. The imaging and motion control platform is designed to maximise optical pathway accessibility and flexibility, while also simplifying the optical alignment.

An advantage of the MicroMirror TIRF system is the open access to the entry and exit optical pathways. The motion control platform comprises an XY micropositioning platform with an integrated XYZ closed loop nanopositioning system. The XYZ closed loop nanopositioning uses proprietary PicoQ® sensors for sub-nanometer precision and high stability. A Z-axis micropositioner with integrated sample holder is mounted above the nanopositioning system.

Completing theMicroMirror TIRF system is the TIRF Lock feedback control consisting of a QPD sensor, TIRF Lock controller and software. The TIRF Lock module maintains the TIR signal through software feedback to the nanopositioner.

Technical Specifications

Imaging & Motion Control  
Micropositioning motion XYZ
Ranges of motion (XY) 25 mm
Range of motion (Z) 10 mm
Body Material Anodised Aluminium
Nanpositioning Motion XYZ
Ranges of motion (XYZ) 200 µm
Resonant Frequency (XYZ) 350 Hz ±15%
Recommended max. load (horizontal)    0.5 kg
Body Material Aluminum
Controller Nano-Drive®
Digital Interface USB 2.0
Analogue Input 0 to 10V
Synchronisation outputs TTL (4)
Nanopositioner Software Nano-Route®3D
MicroMirror Mounts  
MicroMirror motion 6 axis
MicroMirror diameter 2 mm
Mount material Aluminium
TIRF Lock Feedback  
TIRF-Lock Sensor Quadrant Photodiode
Controller TIRF Lock
Digital interface USB 2.0
Software TIRF Lock

A number of articles have been published by scientists around the globe who have used a Mad City Labs Micromirror TIRF within their research. Here is an example:

The Mad City Labs' RM21™ is a versatile precision aligned frame suitable for a wide variety of microscopy techniques such as TIRF



Need stability?

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Go to... Mad City Labs website

Product Code: Mad City Labs MicroMirror TIRF

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