ISS VIVIsight PRO Microscopes

ISS  VIVIsight PRO   

Overview of VIVIsight PRO

ISS introduces the VIVIsight PRO, a compact, affordable, and versatile multiphoton laser scanning microscope specifically engineered as an all-in-one solution for in vivo imaging applications. Harnessing super-fast photon counting techniques with hybrid photomultiplier tube (PMT) detectors, VIVIsight PRO excels in delivering exceptional sensitivity within an expansive dynamic range. VIVIsight PRO seamlessly integrates with a wide array of super-fast femtosecond lasers, ranging from single-wavelength fiber lasers to fully tunable Ti:Sapphire lasers, providing customized yet affordable solutions tailored to your unique requirements.

Multiphoton microscopy relies on the simultaneous absorption of two or more photons to excite fluorophores within the sample. This technique utilizes near-infrared (NIR) laser light, which has longer wavelengths compared to visible light, allowing for deeper tissue penetration. By focusing high-energy pulses of NIR light into a small volume of the sample, multiphoton microscopy selectively excites fluorophores within the focal plane, generating fluorescence signals only from the focal point. This confocal-like optical sectioning reduces out-of-focus background fluorescence and enables high-resolution imaging deep (from hundred micrometers to millimeters) within live tissues and animals. Another benefit of the system is its potential for integrating Fluorescence Lifetime Imaging (FLIM). FLIM can be seamlessly added to the VIVIsight Pro, significantly enhancing quantitative imaging and multiplexing capabilities. This technology has proven to be an excellent tool for metabolic imaging, making it valuable for studying cancer, neurodegenerative diseases, diabetes, cardiovascular diseases, and respiratory conditions.

Whether delving into neuronal dynamics or investigating tumor micro-environments, VIVIsight PRO effortlessly adapts to diverse research needs, ensuring unparalleled versatility in experimental design. Elevate your research endeavors with VIVIsight PRO, where cutting-edge technology meets customizable precision.

Applications for VIVIsight PRO

• Neuroscience: Studying neuronal structure and function in brains, mapping neural circuits, and investigating neurodegenerative diseases.
• Cancer Biology: Visualizing tumor microenvironments, assessing tumor growth, and evaluating responses to therapeutic interventions.
• Developmental Biology: Observing embryonic development, organogenesis, and tissue morphogenesis in live embryos.
• Vascular Biology: Investigating blood flow dynamics, vascular permeability, and angiogenesis in vivo.

3D Volume Rendering

An example of 3D volume rendering with FLIMsight PRO. A stack of z-sectioning images acquired from a pollen grain, using the two-photon laser excitation at the 780 nm wavelength.

Hematoxylin and Eosin-stained Tissue

Image acquired from a hematoxylin and eosin-stained tissue using the two-photon laser excitation at the 780 nm wavelength and a hybrid PMT photon counting detector. Both are capable of fluorescence lifetime imaging (FLIM). Together with ISS FastFLIM technology, it is straightforward to collect the fluorescent decay data at each image pixel. The time-resolved data is quantitatively unmixed by the ISS VistaVision phasor plots module producing three separate images which represent the fluorescence intensity contributed exclusively by distinct fluorescent species. The merged image is constructed utilizing false red, green and blue colors for shortest, intermediate and longest lifetime species, respectively.

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