Fluorescein isothiocyanate Dye Filter Set for Fluorescence applications
FITC Filter Set
This Iridian single band filter set consists of the 475-35 EX, 530-43 EM and 499 DM filters. It is optimised for fluorescein isothiocyanate (FITC) and other fluorophores such as rsGFP, BODIPY, 5-5-FAM, Fluo-4 and Alexa Fluor 490.
The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third-party manufacturers.
| Specifications | 475-35 EX | 530-43 EM | 499 DM |
| Centre Wavelength (nm) | 475 | 530 | |
| Operating Wavelength (nm) | 200 to 1000 | 200 to 800 | |
| Pass Band (nm) | 457.5 to 492.5 | 508.5 to 551.5 | 508 to 675 |
| Transmittance (%) | 90 avg. | 90 avg. |
| Blocking Range 1 (nm) | 200 to 440 | 200 to 496 | |
| Blocking Range 2 (nm) | 505 to 1000 | 575 to 800 | |
| Blocking Level (OD) | 5 avg. | |
| Reflection Band (nm) | | 470 to 490 |
| Reflectance (%) | | > 90 avg. |
| Angle of Incidence | 0° to 5° | 43.5° to 46.5° |
| Cone Half Angle | 7° | 2° |
| Outer Diameter (mm) | 25 +0/-0.2 | |
| Clear Aperture | 21 mm | 80% elliptical |
| Thickness (mm) | 2 ±0.1 | 1.05 ±0.05 |
| Ring mounted | Yes | |
| Ring Thickness (mm) | 3.5 ±0.1 | |
| Width (mm) | | 35.6 ±0.1 |
| Height (mm) | | 25.2 ±0.1 |
| Flatness | | 2λ |
| Parallelism | | 180″ |
| Scratch / Dig | 80/50 | 60/40 |
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Terms and Conditions V6
