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DAPI Filter Set for Fluorescence Spectroscopy

DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
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DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
DAPI Filter Set for Fluorescence Spectroscopy
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  • Model: Iridian DAPI
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DAPI Fluorescent Stain Filter Set for Fluorescence applications   

DAPI Filter Set   

This Iridian single band filter set consists of the 377-60 EX, 447-60 EM and 409 DM filters. It is optimised for 4',6-diamidino-2-phenylindole (DAPI) and other fluorophores such as Alexa Fluor 350, AMCA, BFP, Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue and sgBFP.

The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third-party manufacturers.

Specifications 377-60 EX 447-60 EM 409 DM
Centre Wavelength (nm) 377 447  
Operating Wavelength (nm) 200 to 700 200 to 1000  
Pass Band (nm) 352 to 402 417 to 477 415 to 570
Transmittance (%) 85 avg. 90 avg.
Blocking Range 1 (nm) 200 to 344 200 to 402  
Blocking Range 2 (nm) 409.5 to 700 488 to 1000  
Blocking Level (OD) 5 avg.  
Reflection Band (nm)   327 to 404
Reflectance (%)   > 90 avg.
Angle of Incidence 0° to 5° 43.5° to 46.5°
Cone Half Angle
Outer Diameter (mm) 25 +0/-0.2  
Clear Aperture 21 mm 80% elliptical
Thickness (mm) 2 ±0.1 1.05 ±0.05
Ring mounted Yes  
Ring Thickness (mm) 3.5 ±0.1  
Width (mm)   35.6 ±0.1
Height (mm)   25.2 ±0.1
Flatness  
Parallelism   180″
Scratch / Dig 80/50 60/40
 

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