ISS Evident (Olympus) 3D Nanoimaging & Particle Tracking Upgrade

ISS  FLIM & FCS Upgrade Kits

Overview of Evident (Olympus) 3D Nanoimaging & Particle Tracking Upgrade

Within the past few years, several methods have been devised in order to obtain images with nanometer resolution of cellular features using an optical microscope (STED, PALM, STORM). Although powerful, these methods are quite inefficient when detecting sparse nanostructures in an image. Also they are inadequate to detect the dynamics of chemical reactions which occur in the sub-second time scale in nanometer-size 3D structures, which are continuously moving and changing shape.

With NanoImaging approach to super-resolution, the laser beam does not scan the sample following a predetermined pattern as is the case in raster images. Instead, the laser scanning imaging is based upon a feedback algorithm where the path followed by the laser beam is continuously adjusted and decided during the scan according to the shape of the object to be imaged. The algorithm moves the laser spot at a fixed distance from the object's surface; as the position of the laser spot and its distance from the surface are known parameters, they are utilized to reconstruct the shape of the object. 3D cellular structures can be resolved down to 20 - 40 nm with a precision of 2 nm in a matter of a few seconds.

ISS- link to  website